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Immunoblotting Total cellular protein was extracted using RIPA lysis buffer (10mM Tris-Cl pH 7.5, 150mM NaCl, 1mMEDTA, 1%TritonX-100, 0.1%SDS) supplemented with Roche complete protease inhibitor cocktail (Sigma, 11836145001), PhosSTOP phosphatase inhibitor cocktail (Roche, 4906837001)